Abstract

• In the course of the investigation of endophytic fungi were isolated from the leaf of Annona squamosa L. used PGA (Potato Glucose Agar) and LCA (Low Carbon Agar) media. After isolation, morphology of 7 days old culture were studied. According to this study, 3 different fungi (NA-01, NA-02 and NA-03) were isolated. In the study of antibacterial activities, endophytic fungus (NA-01) showed the highest antibacterial activities against Micrococcus luteus than other fungi. In the present investigation, endophytic fungi was isolated for antibacterial metabolite production, and effect of fermentation medium, pH and temperature variation were optimized for maximum antibacterial metabolite against Micrococcus luteus. The maximum production of antibacterial metabolite was observed in FM-1 medium (glucose, glycerol, yeast extract, peptone, K2HPO4, CaCO3) at pH 5.0 and incubation temperature of 25ºC with shaking condition.