Abstract

• Fungi were isolated from seven different sources. Soil sample was collected from the car workshop as fuel oil contaminated soil, Thuwana Township, Yangon Region, Myanmar. Other samples were collected from pork sausage, cheese, margarine, tea leaves in bean oil with salt, scraped coconut shell and scraped coconut peel. Fungal strains were directly isolated from 6 different sources. Diluted soil (concentration - 10-3 , 10-5 , 10-6 , 10-7 , 10-8 , 10-9 ) was used to culture the fungi. Fungal strains were cultured on Potato Dextrose Agar (PDA) medium. Lipolytic fungi were screened using Tributyrin Agar (TBA) medium. Total twenty-five fungi were observed from seven different sources. Two fungal strains from pork sausage, five fungal strains from cheese, one fungal strain from margarine, two fungal strains from tea leaves in bean oil with salt, seven fungal strains from scraped coconut and eight fungal strains from fuel oil contaminated soil were observed. Among them, ten lipolytic fungi showed clear zone of hydrolysis around fungal colony on TBA medium that indicated lipase enzyme was produced. The isolated fungi were identified by their pure colony morphology and spore formation according to the references. In the present study, ten different types of lipolytic fungi were observed from six different sources.