Abstract

• In the present research, the antifungal compounds isolated from endophytic fungus, Aspergillus duricaulis was carried out by paper chromatography using the solvent 20% NH4Cl, ethyl acetate saturated with water, n-butanol saturated with water, n-butanol-acetic acid- water (3:1:1) for the extraction of antifungal metabolites against Candida albicans. And then, the fungal culture filtrate was studied by the ratio of two solvents, ethyl acetate and n-butanol to fermented broth (1:1, 2:1, 3:1v/v). The equal ratio (1:1 v/v) ethyl acetate extract showed higher inhibitory effect (26.75 mm) than n-butanol extract (20.49 mm). Crude ethyl acetate extract (5.0 g) was obtained from 17 liter of fermented broth and subjected to purification over silica gel column chromatography with various solvent systems. By silica gel column chromatographic separation, compound A (aromatic primary amide, 23 mg colourless crystal) and compound B (aliphatic ester, 24 mg yellow semisolid) in hexane: ethyl acetate solvent system were isolated. These isolated compounds were characterized by Rf value, physicochemical properties, modern spectroscopic methods such as UV and FT IR. In the investigation of minimum inhibitory concentrations (MICs), it was observed that MICs value of antifungal compound A and B were 0.625 µg/mL and 1.25 µg/mL on Candida albicans respectively.