Abstract

• Soil samples were collected from six different areas of Yoar Thit village, Kin Sein Zay and Pauk Myang village of Myint Tha Township, Mandalay Region. These sample were cultured on Flo Medium, Nutrient. Agar medium, CAS medium, Glucose Peptone Agar medium and Centenum medium. The experiments were carried out at the Microbiology Laboratory of Botany Department in Mandalar Degree College. A total of 36 bacterial colonies were obtained from these soil samples. These isolated bacteria were designated as SY-1 to 36. Antimicrobial activity of all strains were carried out by agar well diffusion assay with five test organisms. Among them, SY -12, 13, 17, 22, 27, 29 and 32 strains showed different levels of antimicrobial activities. Especially, SY-17 showed the highest antifungal activity (39.06 mm) on Candida albicans. Therefore, SY-17 was selected and the fermentation condition of this bacterium was carried out by the study of fermentation period 3 days (27.77 mm), proper age 72 hrs (26.02 mm), size 20 % (16.86 mm), different carbon sources maltose (38.05 mm) and nitrogen sources sodium nitrate (48.19 mm), effect of fermentation medium FM 5 (29.98 mm), pH 7 (21.95 mm), temperature 25°C (39.65 mm), and shaking (34.01 mm) and static culture (26.02 mm) on antimicrobial activity against Candida albicans. It result of this work can provide the knowledge of nature of soil bacteria and how to select active strains exhibited against some test organisms.