ISOLATION OF SOIL BACTERIA FROM NGATHAICHAUNG TOWNSHIP AND THEIR ANTIMICROBIAL ACTIVITIES
Abstract
- Soil samples were collected from three different areas of Ngathaichaung Township, Ayeyawady Region. These samples were cultured on Glucose Peptone Agar medium, Flo Agar medium and Dextrose Casein Peptone Agar medium. A total of 15 bacterial colonies were isolated from these soil samples. 5 isolates were obtained from Glucose Peptone Agar medium, 7 isolates from Flo Agar medium and 3 isolates from Dextrose Casein Peptone Agar medium. Isolated bacteria were designated as KM. In the colony morphology, the size of isolated bacteria were medium, large and very large. The margin of colonies were entire, lobate and undulate. Cell morphology of isolated strains were studied by Gram staining, colony morphology and shape of cell strains were short rod, other strains were long rod chain and rod chain. Three strains were Gram positive and other strains were Gram negative. Moreover, antimicrobial activities of all isolated strains were carried out by agar well diffusion assay with seven test organisms. Among them, 6 isolated strains showed the antimicrobial activity. KM-13 showed the higher antibacterial activity (27.77 mm and 27.63 mm) against Escherichia coli and Bacillus pumilus. Especially this strain exhibited the highest antifungal activities (31.17 mm) on Malassezia furfur followed by (25.46 mm) on Candida albicans. And then, KM-11 also showed the antibacterial activity (21.40 mm) on Bacillus subtilis.
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Year
- 2020
Author
-
Khin Min Min Kyaw
Subject
- Botany
Publisher
- Myanmar Academy of Arts and Science (MAAS)