Abstract

• In this research work, one of Myanmar traditional medicinal plants, Protium serratum (Wall.ex Colebr.) Engl., Myanmar named Gati was selected for chemical analysis and pharmacological investigation. The stem bark of the selected plant was collected from Pyin Oo Lwin Township, Mandalay Region. Acute toxicity test of 95% ethanolic extract of the stem bark of the selected plant was examined by Organization of Economic Cooperation and Development (OECD) guideline 425. According to acute oral toxicity test of ethanolic extract of the stem bark of this plant, the test substance can be considered relatively safe to the dose level of 5000 mg/kg body weight. Furthermore, the bioactive compound was isolated from ethyl acetate portion serratum (Wall.ex Colebr.) Engl. by thin layer and column chromatographic method. The yield percent of this pure compound was found to be 1.21 based upon the ethyl acetate crude extract. The molecular formula of this isolated flavonoid compound was determined as C15H14O6 by using some spectroscopic techniques, such as FT IR, 1HNMR, 13CNMR, DEPT, HSQC and DART-Mass spectrometry. The structure of this compound was elucidated by using DQF-COSY, 1HNMR splitting patterns, coupling constant (J values) and HMBC spectroscopic data. IUPAC name of the isolated compound is C2(S), C3(R)-2-(3'-4'-dihydroxyphenyl)- 3,4,-dihydro-2H-chromene-3,5,7-triol.