Abstract

• Six different soil samples were collected from six different places in Pyawbwe Township, Mandalay Region. Thirty different soil fungi SK-1 to SK-30 were isolated from six different soil samples. Isolation of fungi SK-1 to SK-30 were undertaken by serial dilution method. The isolated fungi were cultured on Blakeslee’s Malt Extract Agar (BMEA), Czapek Dox Agar (CZA), Malt Extract Agar (MEA) and Potato Glucose Agar (PGA) media. Antimicrobial activity of isolated fungal strains was evaluated by using the agar well diffusion method with seven test organisms. Among them, three fungal strains SK-1, SK-3 and SK-6 showed the antimicrobial activity against Bacillus subtilis and Candida albicans at 7 days. Especially, SK-6 gave the best antifungal activity against Candida albicans. Therefore, SK-6 was selected and the optimum conditions for antimicrobial metabolite production on Candida albicans of SK-6 were the fermentation period, suitable age and size of inoculum, different carbon and nitrogen sources.