Abstract

• Tumeric (Curcuma longa) has been known to use in Myanmar traditional medicine system in connection with antioxidant, antimicrobial, anticancer and antibacterial activities and therefore locally grown tumeric (Curcuma longa) has been chosen for this study. It is widely used in the world for many purposes and it is also one of the exports of Myanmar. But some turmeric contains aflatoxin and it causes main barrier of earning foreign currencies. Aflatoxin-toxic carcinogenic secondary metabolities are produced by Aspergillus flavus, Apergillus parasititicus and Aspergillus nomius species of fungi. Thus the detection of aflatoxins concentration in food and feeds are very important. High Performance Liquid Chromatographic method was developed and validated according to the protocol on validation of analytical procedures. Analysis of tumeric samples was carried out for aflatoxin B1, B 2, G1 and G2 by HPLC method. Evaluation of radical scavenging activity was detected by DPPH method while contract extraction of aflatoxin was done as per AOAC method with screening by TLC and quantification by HPLC using reference standards. Thus the proposed method is simple, rapid and specific and was successfully employed for quality and quantity monitoring of aflatoxin content in tumeric.