Abstract

• The seeds of Myristica fragrans Hott. (Zadeik-po) are the chief ingredients in a variety of Myanmar Traditional Medicine Formulations (TMF). These formulations are generally used for the treatment of asthma, dysentery, diarrhoea, pneumonia, typhoid fever, hemolytic anemia and urinary tract infection. The Zadeik-po seeds were selected as plant material and collected from Mudon Township, Mon State. The research was focused on antibacterial activity of various extracts and isolation, identification of some organic constituents from Zadeik-po seeds. The polar, non-polar extracts and essential oil of Zadeik-po seeds were screened for antibacterial activity by agar disc diffusion method. The essential oil of Zadeik-po exhibited the pronounced antibacterial action against all tested 20 microorganisms. In addition, Minimum Inhibitory Concentration (MIC) values of active essential oil of Zadeik-po were also determined by microplate dilution method on five bacterial strains. The lowest MIC values of essential oil was found to be 0.0625 mg mL-1 with Escherichia coli LT. α-Pinene (0.5 %), Myristicin (0.03 %) and Eugenol (0.01 %) were isolated from essential oil of Zadeik-po by column and preparative thin layer chromatographic methods. They were identified by UV, FT IR, 1HNMR and GC MS spectroscopic methods. The isolated compounds also showed antibacterial property against the Staphylococcus aureus, Escherichia coli, Shigella boydii, Samonella typhi and Vibrio cholera. From these scientifically observations, it can be inferred that Zadeik-po seeds may be used in the formulation of medicine especially for the treatment of diseases related to bacterial infections.