Abstract

• In this research work, Vanda coerulea Griff. (local name: Vanda Thit-Kwa) was selected for the chemical analysis. The root and stem of this plant contains alkaloids, glycosides, flavonoids, polyphenols, sugars, lipophilic, terpene, saponins and phenolic compounds respectively. The antimicrobial activities of the plant extracts and the pure isolated organic compound were tested by agar well diffusion method using six organisms. The pure organic compound (NNW-1) was isolated from the root and stem of Vanda Thit-Kwa by thin layer and column chromatographic separation techniques. The pure organic compound was obtained as colourless amorphous. The melting point of this compound was found to be 180-182 C. The yields percent of pure compound is 1.408 % (35.2 mg) based upon the EtOAc crude extract. The molecular formula was done by using FT IR, 1HNMR, 13CNMR, DEPT, HMQC spectroscopy and Mass spectrometry and its formula is C24H28O5 and hydrogen deficiency index is 11. In addition, the complete structure of the organic compound (NNW-1) was elucidated by applying 1D and 2D NMR spectroscopy as well as EI-Mass spectrometry. The name of the isolated compound is 3-[(5-(3-hydroxypropyl)-2–propenylphenyl]-2-(4-methoxy-phenoxymethyl) acrylic acid methyl ester.