Abstract

• In this research work, one of the Myanmar medicinal plants, Myrica nagi Thunb. (Local name : Kat pha la) was selected for the chemical analysis. Preliminary detection of phytochemical compounds present in the bark of Kat pha la were carried out according to the test tube method. The percent composition of elements of the dry powder from the bark of Kat pha la was determined by using WDXRF spectrum. The antimicrobial activities of the crude extracts and the pure organic compound (TMK-1) were tested by agar well diffusion method using six organisms (Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus pumilus, Candida albicans and Escherichia coli). The pure organic compound (TMK-1) was isolated from the bark of Kat pha la by thin layer and column chromatographic separation techniques. Melting point and phytochemical test of pure organic compound (TMK1) were recorded. The molecular formula was determined by using FT-IR, 1 H NMR, 13C NMR, DEPT, DQF-COSY, HMQC, HMBC spectroscopy and Mass spectrometry. In addition, the complete structure of terpene derivative compound (TMK-1) was elucidated by applying 1D and 2D NMR spectroscopic techniques as well as EI-Mass spectrometry. Moreover the conformational structure of isolated compound (TMK-1) was examined by using 1 H NMR spectrum, NOESY spectrum and the model study.