Abstract

• In this study, gold-silver bimetallic nanoparticles (Au-Ag NPs) were synthesized by reduction of different volumes of silver nitrate (AgNO3) and chloroauric acid (HAuCl4) using 1 % w/v chitosan solution as reducing agent. The existence of nanoparticles in colloidal solution was confirmed by Tyndall effect. From the UV-vis spectra, the maximum absorptions of Au-Ag NPs were observed at the wavelengths of 479-562 nm which confirms the characteristic of nanoparticles. The antimicrobial activities of nanoparticles against the organisms were evaluated by agar well diffusion method. Based on the results of UV-vis spectroscopy and antimicrobial activities, sample (Au-Ag NPs 2) was chosen and characterized by XRD, FT IR and EDXRF analyses. XRD spectral data indicated that the selected sample was crystalline nature with nanosizes. The corresponding functional groups and the relative abundance of elements in nanoparticles were observed from FT IR and EDXRF analyses. The application of selected sample was also studied in biomedical field especially burn wound healing compared with standard sofratu drug. It was found that bimetallic nanoparticles (Au-Ag NPs 2) was better than sofratu for healing of burn wound with well-developed sebaceous glands, sweat glands and hair follicles in epidermis layer of skin. This research therefore contributes to academics as well as biomedical application.