Abstract

• This research was studied on determination of antioxidant activity, toxicity of some elemental content, and antimicrobial activity of Traditional Eye Medicine, Setkupala (No.2) manufactured by Ministry of Health. In this work, the sample was collected from Myanmar Traditional Medicine shop, Yangon. Percent inhibition of the watery extract was tested by DPPH assay method. IC50 value of the sample was calculated by linear regression excel program. From the results, it is noted that IC50 values of Setkupala (No.2) had slight antioxidant activity. The elemental content of the sample was investigated by EDXRF and AAS methods. Iron content was higher than Selenium, Zinc, and Manganese in the sample whereas the content of Arsenic and Lead were present in the highest toxic elements. The contents of Iron, Arsenic, and Lead in the samples based on one oral dose (five tablets) per day were over the maximum permissible level of WHO standard. In the determination of antimicrobial activity, Bacillus subtilis ( N.C.T.C- 8236), Bacillus pumilus (N.C.I.B-8982), Staphylococcus aureus (N.C.P.C- 6371), Pseudomonas aeruginosa (6749), Candida albicans, and E.coli (N.C.I.B- 8134) strains were used Antimicrobial activity test was done by using agar well diffusion method. According to the study of antimicrobial activity, it was clearly noticed that the different extracts of Setkupala (No.2) were effective against the different strains.T herefore, Setkupala (No.2)had antimicrobial activity. From the phytochemical investigation, it is found that the carbohydrates, glycosides, phenolic compounds, reducing sugars, saponins and tannins were present in the sample. Moreover, the sample was examined by FT IR and HPLC method.