Abstract

• One of Myanmar indigenous medicinal plant, Adina cordiforlia Hook.f (Hnaw) was selected for chemical analysis. A pure organic compound was isolated from the bark of Hnaw by using advanced separation methods such as Thin Layer and Column Chromatography. The yield percent of this pure green crystal compound was found to be 2.35 % based upon the ethyl acetate crude extract. The pure isolated organic compound responds medium activity on Bacillus pumilus and E. coli and low activities against Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans. The molecular formula of the pure compound was determined as C22H22O8 by using some spectroscopic techniques such as FT IR, 1H NMR (500 MHz), 13C NMR (125 MHz), DQFCOSY, HMQC, HMBC and EI-Mass spectral data. Hydrogen deficiency index of this compound is 12. Finally, the complete structure of this pure compound as dimer of 2-hydroxy-4-ethoxy cis-coumaric acid was elucidated by employing DQF-COSY, 1H NMR splitting patterns, coupling constant (J- value) and HMBC spectroscopic studies.