Abstract

• In the present work locally grown T. Chebula (Phan -kha), P. emblica (Zibyu) and T. bellerica (Thit-seint) were selected to make nutraceutical tablets. The selected fruit samples were identified at Botany Department University of Yangon. Then the nutritional values, mineral contents, Physicochemical properties, effect of storage time, phytochemical constituents, antimicrobial activity and cytotoxicity of the prepared tablet samples were determined. The water content of 29.94 %, ash content of 4.00 %, protein content of 1.89 %, fiber content of 2.22 %, fat content of 3.04 %, carbohydrate content of 58.91 %, in the tablet sample energy value of 270.56 kcal/100g and vitamin C content of 44.88 mg/100g were observed. The pH of the prepared sample was 4.20 and the total acidity of tablet contained 2.08 mg/100 g . The effect of storage time on water contents, ash contents, vitamin C contents, total acidity contents and pH of prepared tablet samples were also determined. It was found that, the water content slightly decreased from 1 month to 3 month storage time duration. The ash also slightly decreased and the vitamin C content significantly decreased within 3 months duration time. The total acidity slightly decreased and pH values were not changed with the longer storage time. The mineral contents of prepared tablet samples were determined by ED XRF spectrum. It was found that 0.549 % K, 0.138 % Si, 0.118 % Ca, 0.024 % P, 0.021 % S, 0.008 % Fe, 0.003 % Mn, 0.002 % Rb, 0.002 %Cu, 0.002 % Sr and 0.001 % Zn were present in this sample The preliminary phytochemical tests indicated that various types of secondary metabolites such as alkaloids, flavonoids, glycosides, phenolic compounds, reducing sugars, saponins, terpenoids and organic acids together with  -amino acids and starch were present in the sample. The antimicrobial activity of tablet samples from medicinal plant species has been evaluated in vitro against microorganisms including five bacterial species (Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus pumilus and Escherichia coli) and one fungus species (Candida albicans). In general ethanol and watery extracts of tablet samples exhibited antimicrobial activity. The cytotoxicity of water extracts of nutraceutical tablets was evaluated by brine shrimp cytotoxicity bioassay. The crude watery extract was not cytotoxic to brine shrimp up to maximum dose of 1000 µg/ L. The LD 50 value of standard K2Cr2O7 was <1 µg/L and caffeine was > 1000 µg/L.