Abstract

• The present work was conducted to investigate the phytochemical constituents and antimicrobial activity of fruits of Haplophragma adenophyllum (Wall.) Dop. (Phet-tham) on different microbial strains. From the phytochemical investigation, fruits of Phet-tham showed positive for alkaloids, -amino acids, carbohydrates, flavonoids, glycosides, phenolic compounds, reducing sugars, saponins, steroids, tannins, terpenoids and showed negative results for starch and cyanogenic glycoside. Elemental analysis by EDXRF method revealed that fruits of Phet-tham contained K, Ca and Cl as major elements. By silica gel column chromatographic separation, three terpenoid compounds including lupeol (0.033 %, m.pt.=213-214 C) were isolated from pet-ether extract of fruits of H. adenophyllum. Antimicrobial activities of pet-ether, ethyl acetate, 95 % ethanol, methanol and watery extracts from the fruits of Phet-tham were investigated against six species of microorganisms such as Bacillus subtilis, Bacillus pumilus, Staphylococcus aureus, Pseudomonas areuginosa, Escherichia coli and Candida albicans by agar well diffusion method at Pharmaceutical Research Department (PRD), Yangon. Among all tested crude extracts EtOAc extract exhibited the highest antimicrobial activity with the inhibition zone diameter in the range of 13~33 mm against all microorganisms tested.