Abstract

• Piper betle L. (Kun) is a well-known medicinal plant and widely distributed in Myanmar. In this study the leaves of Piper betle L. were collected from Yamethin Township, Mandalay Region, in the month of June to July, 2016. The extraction were done with 95%, 70%, 50% ethanol, aqueous and fresh juice. Endophytic microorganisms were also isolated from the leaves of Piper betle. Six endophytic bacterial strains and three endophytic fungal strains were obtained. Antimicrobial activities of leaf extract and endophytic microorganisms were tested on the seven pathogenic organisms by paper disc diffusion method. The ethanolic leaves extracts of 95% showed significant effect against acillus subtilis, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. Endophytic bacterial strains T-2 and T-5 showed activities against Candida albicans but endophytic fungal strains did not show the effect against on seven pathogenic organisms.