INVESTIGATION ON SOME PHYTOCHEMICAL CONSTITUENTS AND BIOACTIVITIES OF SEED EXTRACTS OF Mucuna Pruriens L.(KHWE-LEYA)
Abstract
- The research focused on the investigation of some phytochemical constituents of (L.) seed and some of its biological activities. The sample was collected from Ingapu Township, Ayeyawady Region and it was identified at Department of Botany, Hinthada University. Preliminary phytochemical tests have revealed the presence of alkaloids, carbohydrates, flavonoids, α-amino acids, starch, organic acids, phenolic compounds, saponins, glycosides, reducing sugars, steroids and terpenoids in the sample according to test tube methods and TLC profile. The seed sample was found to contain 14.39 % of moisture, 3.43 % ash, 23.22 % of protein, 8.98 % of dietary fiber, 1.68 % of crude fat, 48.30 % of carbohydrate, and 302 kcal /100 g of energy value based on dried sample. The seed sample was found to have relatively highest content of K and P whereas minor components of S, Ca, Fe, Zn, Cu and Mn according to EDXRF analysis. The antimicrobial activities of PE, EtOAc, CHCl3, 95 % EtOH and H2O extracts from seed were screened by agar well diffusion method on six species of microorganisms, namely , , , , and . All extracts of seed sample were observed to possess antimicrobial activity. Among the tested crude extracts, EtOH extract was found to possess the most potent antimicrobial activity (inhibition zone diameter ranged between 14~16 mm). antioxidant activities of 95 % EtOH and watery extracts from seed were assessed by DPPH radical scavenging activity assay. The antioxidant activity of watery extract (IC50= 3.62 g/mL) was found to be higher than ethanol extract (IC50= 4.49 g/mL). From the results of phytochemical constituents, antioxidant and antimicrobial activities of the seed observed in the present study, the seed could be applied as the local health remedy for the local indigenous communities of our country.
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Year
- 2018
Author
-
Myint Myin Khine
Subject
- Chemistry
Publisher
- Myanmar Academy of Arts and Science (MAAS)