Abstract

• d out by burning the fish scales at different temperatures. These biomaterials were proved to be nano-sized hydroxyapatite by using X.R.D.(xray diffraction), F.T.I.R.(fourier transform infrared spectroscopy) and S.E.M.(scanning electron microscope). Purity of the sample was confirmed by T.G.A.(thermo-gravimetric analysis), protein content and Ca/P weight ratio. Porosity in the sample was calculated by image J software. Toxic heavy metal was not detected by mineral analysis. Evaluation of hardness of F.S.HAp-Glass Powder-GIC Composite was done by Mitutoyo micro-hardness testing machine. Hardness of pure Glass Ionomer (Japan) was found to be 3.5 Mohs while that of F.S.HAp-Glass Powder-GIC Composite was 4 Mohs. As the hardness is very near to teeth and skeletal tissue, it is suitable for substitution in tooth defect area.