Abstract

• This research is focused on the evaluation of α-glucosidase inhibitory effect from Myanmar Traditional Medicinal Plants Hydrocotyle rotundifolia Roxb. (Say-myin- khwa). The medicinal plant has been reported to possess the antidiabetic potential. According to the preliminary phytochemical tests indicated the presence of alkaloids, α-amino acids, carbohydrates, flavonoids, glycosides, phenolic compounds, steroids, terpenoids, tannins, reducing sugars, and saponins were present while starch was not detected in this sample. The α-glucosidase inhibitory effect of watery and ethanol extracts on production of glucose from sucrose was determined by using UV-Visible spectroscopy. The 50 % inhibitory concentrations (IC50) of watery and ethanol extracts on α-glucosidase activity were found to be 0.73 and 0.61 µg/mL. From this experiment, it was found that ethanol extract showed higher potency than water extract. However, all of these extracts showed lower potency than that of standard drugs Voglibose (IC50 = 0.32 µg/mL in α-glucosidase inhibitory effect. The antioxidant activity of ethanol and watery crude extracts was investigated by DPPH free radical scavenging assay. The IC50 values of watery and ethanol crude extracts were12.34 µg/mL and 9.33 µg/mL. Since the lower IC50 value, the higher antioxidant activity of the samples occurs. Thus, the ethanol extract showed higher antioxidant activity than that water extract.