Abstract

• In this study, collagen was extracted from chicken feet and its biomedical application was investigated. The chicken feet were collected from poultry Myanmar market. The collagen was extracted from chicken feet by using acid solubilized method. The yield percent of crude was found to be 10.08 %. And then the crude collagen was purified by dialysis method, purified collagen was found to be 8.32 %. Both crude and purified collagens were characterized by using SEM, UV-visible and FT IR analysis. The SEM images of collagen to be regular and uniform with networking of porous on the surface. The absorption bands of crude and purified collagen were found to be near 230 nm in UV spectra. In the FT IR spectra, absorption bands of collagen sample indicated the presence of N-H, O-H, C=O, -CH2, -CH3 groups in the sample. The antimicrobial activity of both collagen samples were determined by agar well diffusion method. In this experiment, both samples were found to be high activities against six microorganisms such as Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus pumilus, Candida albicans and Escherichia coli, which showed that collagen possessed the high antimicrobial activity against all tested microorganisms. The wound healing effect of both collagens was investigated. It was found that the purified collagen was well-developed in healing of burn wound with well-developed sebaceous glands, sweat glands and hair follicles in epidermis layer of skin. This research therefore contributes to academics as well as biomedical application.