Abstract

• In the present study, two compounds were isolated from the fruit of Piccrasma javanica and identified as 1-ethyl-4-methoxy--carboline and 1-ethyl--carboline on the basis of their spectroscopic data; UV, IR, 1H and 13C NMR in conjunction with 2D experiments (COSY, HSQC, HMBC) and comparison with published literature. For the cytotoxic effect of P. javanica fruit ethanol and water extracts; by brine shrimp assay method, the LC50 values were found to be 135 µg/mL for the ethanol extract and 318 µg/mL for the water extract. Moreover, the antioxidant potential of ethanol and water extracts was evaluated by DPPH radical scavenging activity, using ascorbic acid as standard and both extracts showed a good antioxidant activity with IC50 values of 7.71 µg/mL and 12.19 µg/mL, respectively. Total phenol content by Folin-Ciocalteu reagent method gave 43.06 ± 0.5 mg GAE/g for the ethanol extract and 21.96 ± 1.3 mg GAE/g for the water extract. Furthermore, the anti-inflammatory activity of the ethanol and water extracts by protein denaturation method was 44.44 % and 42.15 %, respectively, at a concentration of 1000 g/mL.