Abstract

• The present research work was focused on the identification and extraction of antimicrobial active soil fungus HMF-33. This fungus was isolated by serial dilution method from the soil of Naung Taw village, Homalin Township. The macroscopic and microscopic characters of HMF-33 were observed on the Blaskeslee’s Malt Extract Agar (BMEA Medium), Czapek-Dox Agar (CZA Medium), Malt Extract Agar (MEA Medium), Glucose Ammonium Nitrate Agar (GAN Medium), Potato Glucose Agar (PGA Medium) and incubated for 7 days at room temperature. According to the results, selected fungus HMF-33 was identified as the genus Penicillium sp. In the investigation of fermentation medium (FM), eight kinds of fermentation media was studied by using various carbon and nitrogen sources. Among them, FM 6 showed the higher antimicrobial activity than other fermentation media on seven test organisms. In the results of paper chromatography, n-butanol was the most suitable solvent for extraction of antimicrobial secondary metabolites from fermented broth of HMF-33 and bioautographic assay showed Rf values had 0.92 on Bacillus subtilis and 0.90 on Candida albicans. Furthermore, crude extract 33.0g yielded from 17.5L of fermented broth of Penicillium sp., HMF-33 and showed highly antimicrobial activities (20.53-32.94mm) against (gram positive, gram negative bacteria and fungi) ten test organisms. Crude extract of Penicillium sp. (HMF 33) possessed broad spectrum bioactivity. Therefore, crude extract of Penicillium sp. may be used by the pharmaceutical industries for the production of antimicrobial compounds from local sources.