Abstract

• purgative and so on. In this study, preliminary phytochemical investigation of G. superba revealed the presence of alkaloid, flavonoid, glycoside, carbohydrate, starch, -amino acid, terpenoid, steroid, saponin, tannin, phenolic compound, organic acid and reducing sugar. Cyanogenic glycoside was absent in G. superba. Total alkaloids content (1.11 %) of selected plant sample was determined by using gravimetric method of Harbone. The antimicrobial activities of different crude extracts such as pet-ether, ethyl acetate, ethanol, methanol and watery extracts from G. superba were determined against six microorganisms such as Agrobacterium tumefaciens, Bacillus subtilis, Candida albicans, Escherichia coli, Salmonella typhia and staphylococcus aureus by paper disc diffusion method. According to antimicrobial activity screening, pet-ether extract cannot against all strains of microorganisms. EtOAc and H2O extracts were observed to possess mild antimicrobial activity whereas EtOH and MeOH extracts have pronounced antimicrobial activity against all tested microorganisms. Antiproliferative activity of EtOH and H2O extracts of G. superba (tuber) against A 549 (lung cancer) and Hela (cervix cancer) was investigated by using (3-(4,5-dimethylthiazol-2- yl)-2,5-diphenyltetrazolium bromide or MTT Assay. Antiproliferative activities of all extracts showed strong activities (IC50 ˂20 µg/ mL against two cancer cell lines.