Abstract

• In the present research, the bark of Albizia procera (Roxb.) Benth. (Local name = Thit-phyu) was selected for chemical investigation. Preliminary phytochemical investigation was carried out according to the standard procedures. Antimicrobial properties was evaluated by using agar-well diffusion method on six test microorganisms such as Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus pumilus, Candida albican and Mycobacterium species. Moreover, a pure compound, pale yellow needled shape crystals, was isolated by using separation techniques such as thin layer and column chromatography. The isolated compound was assigned as flavonoid derivative, namely, 2(R), 3(S)-2-(2, 4-dihydroxy phenyl) chroman-3,5,7-triol by using spectroscopic methods, such as FT-IR, 1H NMR, 13C NMR (125 MHz), DEPT, DQF-COSY, HMQC, HMBC, NOESY, and EI mass spectral data. The isolated compound was further confirmed by phytochemical test which gave rise to positive for flavonoid test. The yield percent was calculated as (33.87 mg), (1.07%) based upon the ethyl acetate crude extract and the melting point was 191°C– 192°C. Moreover, the antioxidant activity of isolated flavonoid compound was measured by DPPH (1,1–Diphenyl-2-picryl-hydrazyl) assay. The isolated compound showed the high antioxidant activity.