Abstract

• The focus of the present research, the bacterium Pseudomonas sp, isolated from mangrove soil, Shwe thaung yan Township, Ayeyawady Region. It was carried out by paper chromatography with four solvents system. The bacterial culture filtrate was studied by the different ratio of ethyl acetate and fermented broth (1:1, 2:1, 3:1 v/v). The equal ratio (1:1 v/v) ethyl acetate extract showed higher inhibitory effect (19.79 mm) than the other ratio. Crude ethyl acetate extract 2.0 g was obtained from 10 liters of fermented broth with ethyl acetate (1:1 v/v) and subjected to purification was performed using both thin layer chromatography (TLC) method with various solvents system and silica gel column chromatography techniques. By silica gel column chromatographic separation, compound M-1 (colorless needle shape, 1.1 mg, may be sterol), compound M-2 (colorless amorphous powder, 5.6 mg, may be steroid) and compound M-3 (colorless amorphous powder, 5.8 mg, may be steroid) in chloroform: methanol solvent system were isolated. These isolated compounds were characterized by Rf value, physicochemical properties, modern spectroscopic methods such as UV and FT IR. In the further investigation of minimum inhibitory concentrations (MIC), it was observed that MIC value of antifungal compounds M-1 and M-2 were 1.25 µg/ mL and 0.625 µg/mL respectively but M-3 did not exhibit antifungal activity against Candida albicans in this experimental concentration range from 20 µg/mL to 0.078 µg/mL. In the present result, indicated that the selected bacterium Pseudomonas sp. may be utilized to treat the diseases caused by Candida albicans.