Abstract

• The research was concerned with isolation, purification and partial characterization of antibacterial compounds of isolated endophytic Actinomycetes TG-16 from the stem of Tadehagi triquetrum (L.)H.Ohashi. These experiments were conducted by solvent extraction, thin layer chromatography, silica gel column chromatography, UV and FT IR spectroscopic analyses. The plant sample was collected from Pathein University Campus. The fermented broth of isolated strain TG-16 was carried out by paper chromatography using with four solvents for the extraction of antibacterial metabolites. the Actinomycetes culture filtrate was studied by the different ratio of two solvents. According to this result, the active metabolite, 15 liter was extracted by using n-butanol to fermented broth (1:1v/v). The analysis of separation, purification, and partial characterization of isolated compounds indicated that compound A (aromatic derivatives, 15 mg, colorless crystal) and compound B (steroid derivatives, 14 mg, white amorphous solid). Further evaluation of minimum inhibitory concentration of compounds showed the value of 3.125 µg/mL in compound B while compound A did not exhibit antibacterial activity against E. coli. This study revealed the presence of bioactive compounds in isolated strain TG-16, which may be a promising resource for the discovery of bioactive metabolites against multidrug resistance of E. coli.