Abstract

• In this study, fifteen actinomycetes were isolated from the four soil samples collected at Pwint Phyu Township, Magway Region. Soil samples were carried out from June 2016 to July 2016. The isolation of actinomycetes was undertaken by the method of Chemical Treatment Dilution Method and Physical Treatment Serial Dilution Method. Soil actinomycetes were tested the antibacterial activities with five test organisms by using paper disc diffusion assay method. These isolated actinomycetes showed the antibacterial activities on test organisms. Among them, actinomycete A-01 showed the highest antibacterial activity on Bacillus subtilis (23.41 mm). Therefore, this actinomycete A-01 was selected for further investigation. In the fermentation studies of A-01, it was found that 46 hrs ages and 2% sizes of inoculum were optimized to produce the antibacterial metabolites.