Abstract

• The present study was focused by the growth conditions and fermentation optimization of selected soil bacterium KM-39 against Candida albicans. In the growth of KM-39, carbon and nitrogen sources were used and the excellent growth were found on lactose and yeast extract. The highest antifungal activity of KM-39 was found by the fermentation conditions such as 2 days old culture period, 20% inoculum size, 72 hours age of inoculum, FM-5 at pH 8 and temperature 40ºC. The maximum antifungal activity was obtained by using the maltose in the carbon source and potassium nitrate in the nitrogen source. In the comparison of static culture and shaking culture of KM-39, the antifungal activity of shaking culture was more higher than the static culture on Candida albicans.