IDENTIFICATION AND EXTRACTION OF ANTIBACTERIAL METABOLITES OF SELECTED ENDOPHYTIC FUNGUS SL-37 AGAINST ESCHERICHIA COLI
Abstract
- The present work was focused on identification of selected endophytic fungus SL-37 from Prunus persica (L.) BATSCH., Met-mon leaves, (Mogok Township, Mandalay Region in 2017) and extraction of antibacterial metabolites. According to the result of macroscopic and microscopic characters, SL-37 was identified as Aspergillus sp. According to the paper chromatography values, Aspergillus sp was produced antibacterial metabolites by using ethyl acetate solvents. The equal ratio (1:1 v/v) ethyl acetate extract was showed higher inhibitory effect (23.91 mm) than (1:1 v/v) n-butanol extract (20.15 mm) against E coli. Crude ethyl acetate (5g) was obtained from 20 Liter of fermented broth SL-37. Then the crude extract of SL-37 was adjusted to pH 4, 5, 6, 7, 8, 9 and 10 and pH 6.0 was showed the maximum inhibitory antibacterial activity (23.18 mm) than other pH against E coli. These results suggested that the selected endophytic fungus may be utilized for screening the antibacterial metabolites.
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Year
- 2021
Author
-
Su Su Latt
Subject
- Botany
Publisher
- Myanmar Academy of Arts and Science (MAAS)