Abstract

• G.Don. (Thanat-gyi) was selected for chemical analysis. It was collected in Pyin Oo Lwin Township, Mandalay Region in Myanmar. The phytochemical screening of this plant was determined which gave positive tests for flavonoids, terpenes, glycosides, reducing sugars, polyphenols, tannins, saponins, lipophilic and phenolic compounds. The antimicrobial activities of crude extract of this plant were tested in various solvent systems by Agar well diffusion method on six selected microorganisms:  and . In addition, the acute toxicity test of 95 % ethanolic extract of the bark of G.Don. was done by Organization of Economic Cooperation and Development (OECD) guideline 425 (2008) on albino mice. The pure organic compound (WH-1) was next isolated from the bark of Thanat-gyi by Thin Layer and Column Chromatographic methods. The pure yellowish brown crystal (0.62 %) was obtained based upon the ethyl acetate crude extract. Furthermore, the molecular formula (C16H12O7), molecular mass (316) and complete structure of this pure compound was determined by FT-IR, 1H NMR, 13C NMR, DEPT, HSC, DF-COSY, HMBC and EI-MS spectral data respectively. The IUPAC name of pure organic compound (WH-1) is (E)4-(4-(2-carboxyvinyl)-2-hydroxyphenoxy)-3-hydroxy benzoic acid.