Abstract

• ochemical constituents from aerial parts of the selected medicinal plant: (L.) Kuntze (Nga-yant-padu) which are widely used in Myanmar for the treatment of diseases such as asthma, cough, typhoid, vermifuge, diarrhea and urinary complaint. By silica gel column chromatographic separation method, 0.002% of palmitic acid (A) (m.pt 63-64C), 0.24% of stigmasta-5, 22, 25-triene-3ol (B) (m.pt 151-152C) was isolated from PE extract of whereas separation of defatted EtOAc extract of this plant provided hispidulin (C) (0.36%, m.pt 287-288C), pectolinarigenin (D) (0.064%, m.pt 208-210C) and stigmasterolglucoside (E) (0.05%, m.pt 283284C). The isolated compounds were identified by determination of melting point, some color tests and modern spectroscopic methods. As part of the work on the bioactivity investigation of selected plant, the antiasthmatic action was studied on histamine induced guinea pig trachea . All selected compounds (except palmitic acid) such as stigmasta-5, 22, 25triene-3ol, hispidulin, pectolinarigenin and stigmasterolglucoside were observed to possess 80.2% (P0.01), 73.0% (P0.01), 70.2% (P0.01) and 63.0% (P0.01) of relaxation response on histamine induced guinea pig trachea. Therefore, it can be inferred that selected plant may be useful as a remedy for the treatment of bronchial asthma.