Abstract

• In this research, itis latifolia Roxb., which is one of the Myanmar indigenous medicinal plants known as Chin taung mwe soke was selected for chemical analysis. Firstly, preliminary phytochemical screening of the tuber of Chin taung mwe soke was carried out, which indicated the presence of alkaloid, flavonoid, glycoside, phenol, polyphenol, sugar, saponin, sterol and terpene. The antimicrobial activity of crude extracts in various solvent systems of the tuber of Chin taung mwe soke was determined by agar well diffusion method on six selected organisms. Furthermore, a pure compound was isolated from the tuber of Chin taung mwe soke as needle shape crystal by Thin Layer and Column Chromatographic methods. The yield percent of this compound was found to be 0.61 % (21 mg) based upon the ethyl acetate crude extract and the melting point was (198-199°C). This pure compound gave positive for sterol test. Moreover, antimicrobial activity of this pure compound was rechecked by using agar well diffusion method. In addition, the molecular formula of pure compound could be determined as applying some spectroscopic methods such as FT IR, 1H NMR, 13C NMR and EI mass spectral data. The complete structure of steriodal derivative compound was elucidated by DF-COSY, HMC, HMBC and DEPT spectroscopic method. Finally, the conformational analysis of an organic compound was carried out by using 1H NMR splitting patterns, coupling constant, NOESY spectral data and model studies. The structure of the isolated compound was elucidated as 10, 11, 15-trimethyl-17-(3-methylnona-2-yl)-2, 3, 4, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17-tetradecahydro-1H-cyclopenta [a] phenanthren-3-ol.