Abstract

• In the present research, the leaf of Calotropis gigantea R.Br. (Mayo), Family- Apocynaceae, was chosen for investigation of some phytoconstituents and bioactivity studies such as acute toxicity, antitumor, and larvicidal activities. Acute toxicity of 95 % ethanol extract was studied with the dosage of 2000 mg/kg, 5000 mg/kg body weight in albino mice and no lethality was observed up to fourteen days after administration. The antitumor activities of PE, 95 % EtOH, EtOAc and H2O extracts were screened on Agrobacterium tumefaciens by Potato Disc Assay method and all extracts exhibited antitumor activity after 5 days and 7 days observation. The larvicidal activities (LC50 and LC90 ) of PE, 95 % EtOH, EtOAc and H2O extracts were investigated in the range of 0.0125 to 0.2 g /100 mL by Aedes larvaemethod. The lowest knockdown of Aedes larvae was found at the concentration of 0.0125 g /100 mL of H2O extract. The highest mortality rate (95.60 %) of Aedes larvae at the concentration of 0.2 g / 100 mL was found in the EtOAc extract. The lowest mortality rate (8.80 %) of H2O extract was observed at concentration of 0.0125 g /100 mL. Among the tested four crude extracts, EtOAc extract showed the highest lethal activity (LC50 = 0.0235 g / 100mL and LC90 = 0.1224 g /100mL).