Abstract

• In this research work, Andrographis paniculata plant samples were collected from Magway University Campus, Magway Region. The isolation of endophytic fungi was undertaken by the surface sterilization method and Baiting method. Fourteen fungi was isolated from leaves, stem and root of Andrographis paniculata. All isolated fungi were tested by five kinds of test organisms, Escherichia coli, Pseudomonas fluorescence, Bacillus subtilis, Saccharomyces cerevisiae and Candida albicans by paper disc diffusion assay method. Among them, Fungus TF-02 exhibited only selective antimicrobial activity against Escherichia coli and the highest activity. Therefore, this fungus TF-2 was selected for further investigations such as fermentation for the production of antimicrobial metabolite, identification. In the study of microbial growth phase of TF-2 was observed that between 66hrs and 96 hrs ages of inoculums and 5%, 10%, 15%, 20%, 25%, 30% size of inoculums. In the investigation of ages and sizes of culture, 84hrs cultivation and 25% seed culture could be optimized for fermentation. The highest activity reached at 84hrs and 25% seed culture by using five kinds of fermentation medium, FM-4 at 25ºC showed the best activity on test organism. The extraction of compound was extracted from the fermented broth. The antimicrobial metabolite TF-02 was conducted with eluting solvents, Chloroform (CHCL3), Dicholoromethane (CH2CL2), Toluene and Hexane. The eluting solvent, Dichloromethane (CH2CL2) was chosen by antimicrobial fungus TF-02. This fungus TF-02 was identified as Aspergillus versicolar.