Abstract

• In this research work, eighteen fungi were isolated from nine soil samples collected in Magway Township Magway Region, by using chemical treatment dilution and soil dilution methods. All isolated fungi were tested with ten kinds of test organisms were using paper disc diffusion assay method. In the study of fungus KFO-06, Penicillium rugulosum showed the highest antibacterial activity on Escherichia coli. Therefore, this fungus was selected for further investigation. In the study of carbon and nitrogen utilization of Penicillium rugulosum for the growth, tapioca powder was excellent for carbon source and peanut cake was excellent for nitrogen source. However, it was observed that the highest antibacterial activity with obtained by using glycerol as carbon source and yeast extract as nitrogen source. Six fermentation media were used for fermentation study and it was found that FM-I showed the highest antibacterial activity on test organism Escherichia coli than other medium. In the fermentation studies 72 hrs seed cultures and 15% size of inoculums were optimized for the fermentation.