Abstract

• In this research, various kinds of fungi were screened from flowers of Catharanthu sroseus (L.) G. Don. The plant specimen of C. roseus was collected from Hlaing Township, Yangon Region, near the Oakkyin bus-stop, on the Insein Road. The direct inoculation method was used to isolate different strains of fungi on Potato Glucose Agar medium. Five strains of fungi were found out in this experiment. These strains of fungi were designated as F1, F2, F3, F4 and F5. The morphological and microscopically characters, colony appearances, hyphae, spore shape, appearance of conidiophores, characteristics of spore head, starch hydrolysis test and urease test were carried out at Microbiology Laboratory, Department of Botany, University of Yangon. Isolated fungi F2 from flower of Catharanthus roseus was assumed to be the genus Mucor sp. and F3 was also to be the genus Aspergillus sp. based on their morphological and microscopic characters. Starch hydrolysis and urease tests were also investigated F2, F3 and F5 are positive result of starch hydrolysis. Except F2, all strains were possessed urease activity. All of fungal isolates were cultured on six different agar such as Czapek dox agar (CZA), Potato dextrose agar (PDA), Potato sucrose agar (PSA), Sucrose yeast agar (SY), Yeast malt extract agar (YMA), and Sabouraud’s dextrose agar (SDA) respectively and their reverse color and observed color were recorded. These isolated fungal strains did not grow well on Sabouraud agar medium. Among them potato sucrose medium is the best for growth of these isolates. These media were applied as fermented broth for antimicrobial activities. The antimicrobial activity of fermented broths from all isolated strains were examined on ten test organisms by paper disc diffusion method and fermentation were carried out for 1 day to 10 days. The fermented broths of all isolated strains F1 to F5 selectively showed good antimicrobial activity from 4 to 6 days on eight tests organisms. Secondary metabolite of activity strains were extracted with ethyl acetate (1:1Vol/Vol) and their antimicrobial activity was also investigated on ten tests organism. The crude of five strains showed excellent antimicrobial activity on eight test organisms.