Abstract

• In this research work, one of the Myanmar medicinal plants, Andrographis paniculata (Burn.f.)Wall. ex Nees was selected for chemical investigation of its endophytic fungus. The endophytic fungi from the leaf of the selected medicinal plant were cultivated on water-agar medium under sterile condition and isolation of pure culture was carried out. Selection of the target fungus was done based on chemical screening (spot pattern on TLC). The selected fungus was found to be Alternaria sp. according to morphological studies. After selecting the target fungus, up-scaled fermentation for pure isolated target fungus was performed on M2 medium. After two weeks, the well-grown culture broths were exhaustively extracted with ethyl acetate. Then, the ethyl acetate extract was evaporated to dryness under reduced pressure to obtain brown crude extracts. The resulting microbial extracts were chromatographed to isolate the pure metabolites. The two pure yellow pigment organic compounds bis-naphtho-g-pyrones derivatives and ferulic acid were isolated from the extracts by using various chromatographic techniques. Furthermore, the structure of isolated compounds were deduced by NMR and mass studies.In addition, antimicrobial activities, cytotoxicity assay and the evaluation of antioxidant activitiy for the two pyrone derivatives compounds were also performed.