Abstract

• Eurycoma longifolia Jack is a plant species that belongs to the Simaroubaceae family, and it is also known locally as Bittu Bark in Myanmar, Piak and Tung Saw in Thailand, and Tongkat Ali in Malaysia. The objective of this study was to evaluate the phytochemical constituents and some biological activities of root of E. longifolia. The phytochemical constituents were investigated by the test tube and TLC profiling methods. Some biological activities such as antimicrobial, antioxidant, and cytotoxicity activities were determined by using the Agar Well Diffusion method, DPPH assay and Brine shrimp lethality bioassay. The antioxidant activity of ethanol extract (IC50 = 204.4 µg/mL) was shown to be more potent than watery extract (IC50 = 765.1 µg/mL) of E. longifolia root. The ethyl acetate extract of the sample was found to have more potent antimicrobial activity against all eight tested microorganisms in the inhibition zone diameter range (24 -32 mm) than those of ethanol, methanol, and watery extracts. Moreover, the cytotoxic effect of ethanol extract (LC50 = 233.35 µg/mL), which showed substantial toxicity was slightly more toxic than the positive control of standard potassium dichromate (LC50 = 244.46 µg/mL)