Abstract

• The present research deals with the analysis to isolate organic compounds and examine antidiabetic activity from the aerial parts of the Bacopa monnieri (Byone-hmwe) plant collected from the Twantay Township, Yangon Region. By column and thin layer chromatography, apigenin (AAT-2) and luteolin (AAT-3) were isolated from the active ethyl acetate fraction and identified by using UV, FT IR, 1HNMR, and NMR spectroscopic methods. The spectrophotometric method was used to determine the antidiabetic activity of a drug using metformin, an oral hypoglycemic agent, as a positive control for the inhibition effect on α-amylase and α-glucosidase enzymes. The α-amylase inhibitory effect (IC50= 54.09 g/mL,78.14 g/mL) and α-glucosidase inhibitory effect (IC50 = 62 g/mL, 91.66 g/mL) were observed in ethanol and watery extracts. Furthermore, apigenin and luteolin inhibited -amylase (IC50= 1.049 g/mL, 0.75 g/mL) and α-glucosidase (IC50 = 0.558 g/mL, 0.507 g/mL), which was comparable with the standard drug metformin (IC50 = 0.497 g/mL). The percentage of enzyme inhibition of isolated luteolin was nearly the same as that of standard metformin.