Abstract

• The aim of this research is to isolate bioactive compounds and to investigate some biological activities, such as antioxidant, -amylase and antimicrobial activity, of the fruits, seeds, bark and leaves of Ziziphus mauritiana Lam. (Zee). According to the phytochemical tests, Zee bark and leaves have more secondary metabolites than other. By thin layer and silica gel column chromatographic methods, lupeol was isolated from the ethyl acetate extract of the seeds and identified by the nuclear magnetic resonance (NMR) spectroscopic method. The antioxidant activity of bark of ethanol extract (IC50 = 2.51 μg/mL) and watery extract (IC50 = 3.30 μg/mL) was found, and Zee leaves of ethanol extract (IC50 = 3.10 μg/mL) and watery extract (IC50 = 2.11 μg/mL) were detected. In -amylase activity, the percent inhibition of α-amylase activity of ethanol extracts of fruits, seeds, bark and leaves has good activity, but water extracts have mild activity. According to antimicrobial activity, the ethanol extracts of all four portions have good activity.