Abstract

• The thermophilic bacterial strain No. 21 which was obtained from the Department of Biotechnology, Mandalay Technical University, showed antagonistic activity against S. aureus (MRSA). Ethyl acetate crude extract was prepared from the strain fermentation broth. A pure Organic compound (SMOo) was isolated as a colourless oily form (250 mg) from ethyl acetate extract by chromatographic separation methods. The chemical structure of the isolated compound was identified by using modern spectroscopic techniques such as FT IR, 1H NMR, COSY, 13C NMR, HMQC, DEPT, HMBC and EI-MS. In addition, the antibacterial activity of the isolated compound was investigated by the agar well diffusion method and the isolated compound showed significant inhibitory activity on S. aureus (MRSA).