Abstract

• The present work deals with the investigation of phytochemical screening, nutritional values and some biological activities of Byttneria pilosa Roxb. (Sat-le-pyat roots, leaves and stems). The plant was collected from Kyauk Taw Township, Rakhine State, Myanmar. The root of this plant is chewed or juice is tropically applied as antidote in case of poisoning. Paste prepared from tender stem with leaves is tied to around limbs for the treatment of fractured bones by the Khumi community. According to the phytochemical investigation, sat-le-pyat roots, leaves and stems revealed the presence of alkaloids, starch, phenolic compounds, flavonoids, carbohydrates, &-amino acids, glycosides, tannins, saponins, terpenoids and steroids and the absence of reducing sugars and cyanogenic glucosides. The nutritional values of Sat-le-pyat plants showed that carbohydrate (66.18 %) and fiber (10.35 %) in roots, carbohydrate (52.55 %) and protein (17.39 %) in leaves and carbohydrate (46.11 %) and fiber (30.44 %) in stems were present as major nutrient than others. In the present chemical investigation, β-sitosterol was isolated from ethyl acetate extract of Sat-le-pyat roots by applying column and thin layer chromatography. The isolated compound was identified by FT IR and physicochemical properties. The antimicrobial activities of PE, CHCl3, EtOAc, 95 % EtOH, MeOH and H2O extracts of roots, leaves and stems were tested against six different microorganisms such as Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus pumilus, Candida albicans and Escherichia coli by agar well diffusion method. The result of antimicrobial activity revealed that EtOAc extracts gave significant activity against all species of microorganisms (12 30 mm) but H2O extract exhibited low activity against all species of microorganisms (11 12 mm) except Bacillus subtilis, Bacillus pumilus and Candida albicans. The antioxidant activity of ethanol and aqueous extracts was determined by free radical scavenging DPPH assay method. From the results, the antioxidant property of aqueous extract (IC50=12.20 μg/mL) was higher than ethanol extract (IC50=48.61 μg/ mL) in roots. The antioxidant power (IC50) of aqueous extract and ethanol extract of leaves was found to be 15.09 μg/mL and 19.59 μg/mL. And also, the antioxidant property of ethanol extract (IC50=11.79 μg/mL) showed higher activity than aqueous extract (IC50=29.34 μg/mL) in stems.