Abstract

• The silver carp Hypophthalmichthys molitrix and silver barb Barbonymus gonionotus belonging to family Cyprinidae were collected from Thatyetkone Fisheries Station, Mandalay Region, during January to August, 2022 to evaluate the mitotic check of cellular process. Various colchicine concentrations (CC) were injected below the pelvic fins depend on the fish weight (1 ml/100g). Liver, oral cells, kidney, heart, gill filaments and blood cells were extracted and treated with hypotonic solution (HS) 0.56 % KCL for various durations. In silver carp, the highest frequencies of metaphase stage 100 % (n=22) in kidney treated with CC 0.50 % for 5 hrs with HS for 1 hr, 81.25 % (n=13) in liver treated with CC for 3 hrs by exposing HS for 45 mins, 75.00 % (n=18) in heart with CC for 4 hrs and HS for 1 hr, and the lowest 47.83 % (n=11) in kidney with CC for 5 hrs with HS for 1 hr 30 mins. In silver barb, the highest frequencies of metaphase stage were 88.14 % (n=52) in gill filaments treated with CC 0.10 % for 2 hrs with HS for 10 mins, 79.17 % (n=57) in kidney treated with CC 0.10 % for 2 hrs with HS for 30 mins, 88.10 % (n=37) in gill filaments treated with CC 0.05 % for 5 hrs by exposing HS for 1 hr, 30.77 % (n=32) in kidney with CC for 6 hrs and HS for 1 hr, 12.50 % (n=8) in blood cells with CC for 4 hrs 45 mins with HS for 1 hr and the lowest 4.94 % (n=4) in oral cells with CC 0.30 % for 2 hrs with HS for 2 hrs 45 mins. These results will provide the foundation of genetic assessment in mitotic cell division of freshwater fishes.