Abstract

• obial, radical scavenging and cytotoxic activities of Sular-na-phar (ldenlandiacorymbosa L.) plant and its bioactive compound were investigated. The antitumor activity of the H2O and EtOH extracts of Sularna-phar was examined by using PCG (Potato Crown Gall) test with the isolated bacterium A. tumefaciens and only H2O extract prevented the formation of tumor. The antimicrobial activity of the crude extracts from this plant sample was determined against six strains of microorganisms by agar well diffusion methods at PRD (Pharmaceutical Research Department). The pet ether extract of Sular-na-phar did not show the antimicrobial activity. However, the EtOH and H2O extracts possessed medium and EtOAc extract possessed high antimicrobial activities. Radical scavenging activity of EtOH and H2O extract samples was determined by 1, 1-diphenyl2-picrylhydrazyl (DPPH) Assay. EtOH extract showed stronger radical scavenging activity than H2O extract of plant sample. Cytotoxic effect of EtOH and H2O extracts of whole plant of Sular-na-pharwas investigated against Artemiasalina(Brine Shrimp). The cytotoxic effect of plant extracts was not found on Brine Shrimp up to maximum dose of 1000 g/mL. The bioactive compound(white colour crystal, 0.0285%) was isolated from defatted MeOH extract of . corymbosa (Sular-na-phar) on the silica gel column chromatographic separation. The isolated compound was identified asursolic acid by physico-chemical properties and modern spectroscopic techinques such as UV, FT IR, 1HNMR, 13C NMR spectrometry as well as by comparing with the reported data. Finally, invitro cytotoxicity was also screened by using MTS Cell Proliferation Colourimetric Assay, against H1299, Clone no. 9, A549 and MCF-7 cell lines. According to the results, cell viability cannot be inhibited by ursolic acid for H1299 and Clone no. 9 cell lines significantly. However, the cell viability of A549 and MCF-7 cell can be effectively inhibited by ursolic acid.