Abstract

• In this research work, stem of Coccinia corifolia Cogn. (Kn-pon) and bark of Dolichanrone serrulata Seem. (Tha-khut) were collected from Yekyi Township, Ayarwaddy Region for the investigation of chemical and biological properties such as antimicrobial activity, antioxidant activity, anti-arthritic activity and antitumor activity due to lack of scientific report on these two locally cultivated medicinal plants. Relative abundances of elements analysed by EDXRF showed the presence of calcium, potassium, sulphur, manganese, iron, stronium, zinc and copper in the stems of Kinpon and calcium, potassium, sulphur, zinc, copper and rubidium in the barks of Tha-khut. Determination of nutritional values has also screen carried out by AOAC method resulting moisture (10.69 %), ash (11.81 %), protein (8.71 %), fiber (36.90 %), fat (0.89 %), carbohydrate (31.00 %) and energy value (169 kcal/100 g) in stems of Kin-pon and moisture (11.25 %), ash (9.55 %), protein (2.29 %), fiber (36.17 %), fat (0.08 %), carbohydrate (40.66 %) and energy value (173 kcal/100 g) in the barks of Tha-khut. In the stems of Kin-pon and the barks of Tha-khut, alkaloids, -amino acids, carbohydrates, flavonoids, glycosides, organic acids, phenolic compounds, reducing sugars, saponins, starchs, tannins, terpenoids and steroids were found to be present according to preliminary phytochemical tests. However, cyanogenic glycosides were not found in these samples Total phenol contents, total flavonoid contents and reducing ability of the ethanol extracts have been respectively determined by using Folin-Ciocalteu (F-C) method, Kiranmai et al. method and Oyaizu method in the ethanol crude extracts from the selcted samples. In addition, antimicrobial activity screening was done on various crude extracts by agar well diffusion method against on Bacillus subtilis, Staphylococus aureus, seuomonas aeruinosa, Bacillus pumilus, Cania albicans an schericha coli. It was observed that EtOAc extract exhibited higher antimicrobial activity than other extracts in the stem of Kin-pon whereas EtOH extract showed higher potency in antimicrobial activity than other extracts in the bark of Tha-khut. The antioxidant activity of ethanol and watery crude extracts of the samples was investigated by using DPPH free radical scavenging assay, resulting in the order of Tha-Khut bark (EtOH extract) (IC50 = 42.71 g/mL) Kin-pon stem (EtOH extract) Tha-khut bark (watery extract) Kin-pon stem (watery extract) in antioxidant activity. Antitumor activity screening by Potato Crown Gall (PCG) test revealed that the EtOH and H2O extracts of stem of Kin-pon and bark of Tha-khut possess the tumor inhibition. Furthermore, antiarthritic activity of the ethanol crude extracts has been studied according to their proteinase inhibitory action and inhibition of protein denaturation action. In antiarthritic activity, ethanol extract of bark of Tha-khut showed proteinase inhibitory action and inhibiion of protein denaturation, however that of stem of Kin-pon did not show activities.