Abstract

• Township, Kayin State and verified its botanical name at Department of Botany, Hpa-an University. Preparation of silver nanoparticles and characterization of prepared silver nanoparticles by X-ray diffraction are described in the present work. A cost effective and environmental friendly method has been carried out for phytosynthesis of silver nanoparticles (AgNPs) from different volumes (2, 4, 6, 8 and 10 mL) of silver nitrate solution (1.0 mM) as metal precursor by using different amounts (5, 10, 15, 20 and 25 μL) of Ocimum sanctum L. leaf extract as reducing agent without using other chemicals of reducing agents. Based on the volume of metal precursor solution, the phyto-synthesized silver nanoparticles were designated as AgNP-2, AgNP-4, AgNP-6, AgNP-8 and AgNP-10 for further characterization. From the X-ray diffraction characterization, it was observed that the XRD patterns of AgNP-8 and AgNP-10 from synthesized AgNPs only showed the optimal crystallite aggregates for cubic crystal system. Comparing with (JCPDS, DB card No. 00-006-0480) reference, the peaks at 2 values of both AgNP-8 and AgNP10 corresponded to the Miller indices ( ) of cubic system of Ag nanoparticles. From indexing XRD patterns of cubic system, it could be confirmed that the optimal phyto-synthesized AgNP-8 and AgNP-10 were face-centered cubic (FCC) crystal system because of having Miller indices with all odd or all even. It was observed that the average crystallite sizes of AgNP-8 and AgNP-10 were found to be 57.65 nm and 49.82 nm by using Scherrer formula.