Abstract

• An Endophytic fungal strainAspergillussp. was isolated from the rhizome of ZingibercassumunarRoxb. For the extraction and isolation of the bioactive compounds, 10L fermentation of strain Aspergillussp. and antimicrobial activity of fermented broth with six test organisms were carried out. After fermentation, the filtrate was extracted with methanol on Ambilite XAD 16 resin column at Microbiology Laboratory, Department of Botany, Universityof Yangon. The methanol extract showed high antimicrobial activity against Bacillus subtilis, Candida albicans,Escherichia coli,Malassezia furfur, Salmonella typhi and Staphylococcus aureus. Isolation of the bioactive compounds from the methanol extract was carried out by using silica gel and Sephadex LH20 gel columns with various solvent systems at Department of Organic Chemistry, Ramkhamhaeng University, Bangkok, Thailand. The isolated compounds were characterized by FT-IR spectra, 1D-NMR (1H-NMR, 13C-NMR) and 2D-NMR spectra.The compounds 1, 2 and 3 were identified as 4-amino-1-(1,3-dihydroxy-1- (4-nitrophenyl) propan-2-yl)-1H-1,2,3-triazol-5(4H)-one; 3,6-dibenzyl-3,6-dimethyl piperazine-2,5-dione and aspergillitine. Antimicrobial activity of all isolated compounds was evaluated on six test organisms and showed antibacterial activity onBacillus subtilis,Escherichia coli and Xanthomonasoryzae.