Abstract

• Soil samples were collected from ten different places of Pyin-Oo-Lwin Township, Mandalay Region, Myanmar. These samples were cultured on Rhizobium Medium (RI) and Yeast Mannitol (YM) agar medium . A total of 50 bacterium colonies were isolated from these soil samples and 31 isolated were obtained from RI Medium and 19 isolated from YM Medium. Isolated bacteria were symbolized as KM. In the colony morphology, the isolated bacteria were small, medium and large in size and color were white, yellow, pale yellow, red and brown. The margins were entire, undulated, circled, lobate and rhizoid and the elevations were raised and flat. Cell morphology of isolated strains were studied by gram staining, colony characters and shape of cell. Thirty nine bacterial strains were short rod and four strains were cocci, seven strains were rod. All isolated bacteria were Gram-negative except ten other strains were positive. All these strains were tested for preliminary study of antimicrobial activity and these strains showed the different levels of antimicrobial activity against ten test organisms. Among them, nine isolates showed the different antimicrobial activity. Especially, KM-40 showed the highest antimicrobial activity (25.99 mm) followed by KM-22 (22.73 mm) on Bacillus subtilis respectively.