Abstract

• In vitro propagation of Eucalyptus citriodora Hook. was carried out in the tissue culture laboratory of Vegetable and Fruit Research and Development Centre (VFRDC), Yemon, Hlegu Township, Yangon Region. In multiplication, the shoot apex and nodal segment of Eucalyptus citriodora Hook. were cultured in modified (Murashige and Skoog, 1962) MS medium containing various combinations of naphthalene acetic acid (NAA) and 6-benzylaminopurine (BAP). Among them, EuS6 (NAA 0.75 + BAP 3.0 mg L-1) showed the longest shoot length (2.25 cm) but maximum number of shoot (8.97) and maximum number of leaves (16.74) were obtained from EuS5 (NAA 0.15 + BAP 3.0 mg L-1). It is therefore EuS5 (NAA 0.15 + BAP 3.0 mg L-1) was regarded as the suitable treatment for shoot multiplication. In vitro shoot elongation, micro shoots (1 - 1.5 cm) were sub-cultured on MS medium added on BAP (0.1 mg L-1) and the combination of BAP (0.1 mg L-1) and (Gibberellic acid) GA3 (0.1, 0.5 and 1.0 mg L-1). Among them, BAP (0.1 mg L-1) gave the longer shoot length than the combination of BAP and GA3. Therefore, individual BAP treatment was assumed as a suitable treatment for Eucalyptus shoot elongation. In rooting experiment, half-strength MS medium supplemented with various concentrations of (indole-3-butyric acid) IBA (0, 0.5, 1.0 and 1.5 mg L-1) and also supplemented with sucrose (0, 2, 4, 6 and 8 %) on elongated shoots (1.5 - 2.5 cm). The maximum number of root (1.7) was obtained from 2% sucrose.